APA
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Stejskalová, A., Calderon, K., Collins, M., Feitor, J. F., Ghose, D., Tang, S., … Ingber, D. E. (2026). Human fallopian tube-on-a-chip for preclinical testing of non-hormonal contraceptives with living human sperm. BioRxiv.
Chicago/Turabian
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Stejskalová, A., K. Calderon, M. Collins, Jessica F. Feitor, Debraj Ghose, Shiyan Tang, O. Gutzeit, et al. “Human Fallopian Tube-on-a-Chip for Preclinical Testing of Non-Hormonal Contraceptives with Living Human Sperm.” bioRxiv (2026).
MLA
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Stejskalová, A., et al. “Human Fallopian Tube-on-a-Chip for Preclinical Testing of Non-Hormonal Contraceptives with Living Human Sperm.” BioRxiv, 2026.
BibTeX Click to copy
@article{a2026a,
title = {Human fallopian tube-on-a-chip for preclinical testing of non-hormonal contraceptives with living human sperm},
year = {2026},
journal = {bioRxiv},
author = {Stejskalová, A. and Calderon, K. and Collins, M. and Feitor, Jessica F. and Ghose, Debraj and Tang, Shiyan and Gutzeit, O. and Badey, N. and Gulati, Aakanksha and López, María V. and Chou, David B. and Petrozza, J. and Plebani, R. and Junaid, A. and Budnik, Bogdan and Ingber, Donald E.}
}
The fallopian tube serves as a sperm reservoir, and it is the site where the oocytes become fertilized. Here, we describe development of an organ-on-a-chip microfluidic model of the fallopian tube (FT Chip) lined by primary human epithelial cells and stromal fibroblasts derived from the FT ampulla. Abundant tissue folds lined by hormone-responsive, epithelial cells resembling those seen in vivo formed on-chip, but not in epithelial organoids cultured in gel cultures. Comparative time-resolved analysis of human sperm versus oocyte-sized microparticles introduced into the epithelial channel in the presence of estradiol revealed that sperm movement was significantly reduced, while the oocyte-sized particles increased, relative to movements in acellular chips. When the non-hormonal contraceptive TDI-11861 was administered to the chip, dose-dependent inhibition of human sperm motility was detected. Thus, this FT Chip may offer a human preclinical tool to study FT physiology and assess the efficacy and mechanism of action of contraceptives.